LOGADS24-KP Dermaptera

April 9, 2024

Sample information
Picture
Photos by: Katey P.
Location
Collection date 01/24/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

I collected my insect of choice on January 24, 2024 at 11:50am on the Georgia Southern Armstrong campus. I found my insect by digging through some dirt near the dorms, I captured him in a small container and then tossed him into the vile provided where I watched him die in the liquid provided by our professor.
Putative identification Arthropoda Hexapoda Insecta Dermaptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I completed all steps of gel electrophoresis correctly, along with accurate pipetting skills. I truly believe my results are accurate and I do not believe that my insect is infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Dermaptera was found to be negative for Wolbachia.
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