Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/24/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | I collected my insect of choice on January 24, 2024 at 11:50am on the Georgia Southern Armstrong campus. I found my insect by digging through some dirt near the dorms, I captured him in a small container and then tossed him into the vile provided where I watched him die in the liquid provided by our professor. |
| Putative identification | Arthropoda Hexapoda Insecta Dermaptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I completed all steps of gel electrophoresis correctly, along with accurate pipetting skills. I truly believe my results are accurate and I do not believe that my insect is infected with Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Dermaptera was found to be negative for Wolbachia. |
Water Boatman