Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/23/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collected on 1/23/2024 at around 11:55am. Area surrounded by trees, tall grass, and bushes; chilly but humid, cloudy, and misting rain. Collected in front of the Georgia Southern University Armstrong Police Campus under a bush and trees with a net, forceps and a glass vial with ethanol in it. |
| Putative identification | Arthropoda Hexapoda Entognatha |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Controls worked appropriately, so because of this I know they weren’t contaminated. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Entognatha was found to be negative for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)