LOGADS24-DP Coleoptera

April 9, 2024

Sample information
Picture
Photos by: Destinee P.
Location No map location data available.
Collection date 01/23/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found on a magnolia tree leave at eye-level, slightly damp from rain, humid outside.​

Collected by tearing leaf off of tree and shaking into net.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Coccinellidae

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

When measuring concentration, I calculated a value of 2.08. When looking over gel electrophoresis results, all controls were accurately displayed. Other students in my class with Coleoptera insects also had positive results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coccinellidae was found to be postive for Wolbachia.
Report Inappropriate Post