Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/23/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | The specimen was collected at around 11:00 to 11:30 am using the net sweeping method and placed in a jar. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA-extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | Results in gel electrolysis were accurate, however, result for the collected specimen (SAMPLE 1) was faint but present. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be postive for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)