LOGADS24-JS-Coleoptera

April 9, 2024

Sample information
Picture
Photos by: Jada'Marie S.
Location
Collection date 01/23/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

The specimen was collected at around 11:00 to 11:30 am using the net sweeping method and placed in a jar.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA-extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Results in gel electrolysis were accurate, however, result for the collected specimen (SAMPLE 1) was faint but present.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be postive for Wolbachia.
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