Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/23/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collected: January 25, 2025 Time of collection: 4:35pm Location: grass and near aquatic vegetation Environment: Found under a rock near lots of trees, climate was warm with sun and humid due to previous rain fall Method: Used a container and let the insect crawl into it. |
| Putative identification | Arthropoda Hexapoda Insecta Dermaptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am confident in my results. My controls did work and there was no positive line for Wolbachia in my insect. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Dermaptera was found to be negative for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)