April 9, 2024

Sample information
Picture
Photos by: Kayleigh B.
Location
Collection date 01/23/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected from under the mulch and dirt. Pinecones were also shaken out.
Putative identification Arthropoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The Wolbachia band indicating it is positive is bright and is at the 500 bp. The negative, positive, and no template control worked accordingly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Blattodea was found to be postive for Wolbachia.
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