MAC.AE.SP24#5 Isoptera

Sample information

Photos by: Janine D.
Location No map location data available.
Collection date 01/23/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University

Date of Collection: January 23, 2024

Time of Collection: 2:46pm

Location: One of the trees in the Science building parking lot

Environment: A tree, if not for the large parking lot it would have been a forest or woods.

Method of Collection: Stick and Sheet; hitting the branches

Putative identification Arthropoda Hexapoda Insecta Isoptera


Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used


Wolbachia presence No
Confidence level High
Explanation of confidence level

From resources that I used to do research on the possibility of my Isoptera sample having Wolbachia from Armstrong Campus, the chance is close to none being at 17% locally and 50% from other sources. And with the results from PCR and Gel Electrophoresis coming out with no 16s rRNA gene present, the results back up my original prediction that my Isoptera specimen is Negative from Wolbachia.  

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isoptera was found to be negative for Wolbachia.
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