Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 01/23/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Date of Collection: January 23, 2024 Time of Collection: 2:46pm Location: One of the trees in the Science building parking lot Environment: A tree, if not for the large parking lot it would have been a forest or woods. Method of Collection: Stick and Sheet; hitting the branches |
| Putative identification | Arthropoda Hexapoda Insecta Isoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | From resources that I used to do research on the possibility of my Isoptera sample having Wolbachia from Armstrong Campus, the chance is close to none being at 17% locally and 50% from other sources. And with the results from PCR and Gel Electrophoresis coming out with no 16s rRNA gene present, the results back up my original prediction that my Isoptera specimen is Negative from Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Isoptera was found to be negative for Wolbachia. |
Water Boatman