MAC.AE.SP24#5 Isoptera

Sample information
Picture	Photos by: Janine D.
Location	No map location data available.
Collection date	01/23/2024
Captive / Cultivated?	Wild-caught
Group	Georgia Southern University
Observations	Date of Collection: January 23, 2024 Time of Collection: 2:46pm Location: One of the trees in the Science building parking lot Environment: A tree, if not for the large parking lot it would have been a forest or woods. Method of Collection: Stick and Sheet; hitting the branches
Putative identification	Arthropoda Hexapoda Insecta Isoptera
Methods
Extraction kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes	DNA extraction kit of in-house reagents was used
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	From resources that I used to do research on the possibility of my Isoptera sample having Wolbachia from Armstrong Campus, the chance is close to none being at 17% locally and 50% from other sources. And with the results from PCR and Gel Electrophoresis coming out with no 16s rRNA gene present, the results back up my original prediction that my Isoptera specimen is Negative from Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Isoptera was found to be negative for Wolbachia.

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