MAC.AE.SP24.20 Dermaptera

April 9, 2024

Sample information
Picture
Photos by: Sophia F.
Location
Collection date 01/23/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • insect collected at 2:57 pm
  • Location of Collection: International Gardens at Georgia Southern University (Armstrong Campus)
  • Habitat: rotting tree stump
  • Method of Collection: metal pan, forceps, and plastic bottle
  • Weather Conditions: Overcast, humid
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

I was not confident my insect would have Wolbachia due to the lack of information on Dermaptera and Wolbachia. I also have low confidence because my DNA is not present on the Gel electrophoresis image. My sample shows little to no DNA. There may have been a mistake regarding the introduction of my sample DNA into the gel.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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