Halyomorpha halys

April 24, 2024

Sample information
Picture
Photos by: Fatima A.
Location
Collection date 09/05/2023
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2023 students
Putative identification Arthropoda Hexapoda Insecta Hemiptera Pentatomidae Halyomorpha Halyomorpha halys

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The water control had a DNA band indicating contamination, while DNA+ was empty perhaps indicating a mislabeling error.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
AAAATGCTTGACATTTTTTCGTGTGTGCTCCAACCTCCTGATGGGTCAAAAAAAGAGGTATTAAAATTTCGGTCAGTTAATAGTATAGTAATAGCACCTGCTAACACAGGTAAGGATAATAACAGAAGAAGTGCAGTAATTCCTACTGACCACACAAACAATGGGATTCGTTCAGGGGTTATACCTGTTGGTCGTATATTTATAATAGTTGAAATAAAATTTACTGCCCCTAAAATTGATGAAACCCCAGCCAAATGTAAGGAAAAAATAGCTAAATCAACTGATGCTCCTCTATGTGAGATATTACTTGATAAGGGGGGATAGACTGTTCATCCTGTCCCAGCTCCTGATTCTGCTATTCTTCTTATTAATAATAAAGTTAATGAAGGGGGTAATAATCAGAATCTTATATTATTTAATCGTGGGAAGGCTATATCAGGTGCTCCAATTATTAAAGGGACTAATCAATTACCGAATCCTCCAATTATGATTGGTATTACTATAAAGAAAATTATTACAAATGCATGTGCTGTTACAATTACATTATAAATTTGATCATTACCAATAAATCTTCCAGGCTGTCCTAATTCGATACGGATAATTAATCTTATAGCTGATCCTACTATTCCAGATCATATTCCGAAGATAAAGTATAGAGTTCCAATATCTTTATGTATTAGGTTGCCCACA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Halyomorpha halys was found to be negative for Wolbachia.
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