Sample information
Picture	Photos by: Aditya N, Mukundh M, Sam P, Ajay M
Location
Collection date
Captive / Cultivated?	Wild-caught
Group	Acton-Boxborough Regional High School
Observations
Putative identification	Arthropoda Crustacea Malacostraca Isopoda
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Isopoda was found to be negative for Wolbachia.