Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 09/22/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Acton-Boxborough Regional High School |
| Observations | This Strawberry Seed Beetle was caught in the woods near our school. The cup used to catch the beetle was embedded in the ground and surrounded by lots of leaves which fell from the trees. It’s unclear exactly when the beetle was caught, but the week or so the trap was set out was during autumn. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Carabidae Harpalus Harpalus rufipes |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Harpalus rufipes was found to be negative for Wolbachia. |
Water Boatman