Group 5 CAMS 2024 – Wolbachia in the 90250

May 24, 2024

Sample information
Picture
Photos by: Diego DL
Location
Collection date 05/22/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Came toward the smell of banana peel and day-old eaten apple.  Very large fly, buzzed very loud, and was seemingly easy to catch with petri dish.
Putative identification Arthropoda Hexapoda Insecta Diptera Drosophilidae

Methods
Extraction kit none
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

100 volt, 90 minutes.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Lane 1 (whichever side of the gel has an instant dye front), contains control, and the second lane contains this arthropod. The fourth lane also contains the positive Wolbachia, where they all have a band at the same distance.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Drosophilidae was found to be postive for Wolbachia.
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