Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/14/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | Arthropod caught on tree by lunch benches at CAMS |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Bibionidae Dilophus |
Methods |
|
| Extraction kit | tweezers |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | UView |
| Gel images |
|
| Protocol notes | Both gels ran for just over an hour, before being examined for bands |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | The Wolbachia gel produced almost no evidence. The bands were either too faint to read or the DNA ran off the gel, presumably because of a PCR error causing the Wolbachia samples to not be amplified enough. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman