Dog Flea

November 18, 2020

Sample information
Picture
Photos by: Elijah D.
Location
Collection date 09/16/2020
Captive / Cultivated? Wild-caught
Group St. John High School USD 350
Observations

The flea was captured off of my dog in mid September. He had been under some trees.
Putative identification Arthropoda Hexapoda Insecta Siphoneptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The flea was stored in 90% ethanol for about two months prior to extraction.

DNA Extraction: The DNA was extracted from the whole arthropod. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.

PCR: Mini One Taq polymerase was used.

Gel Electrophoresis:  Mini PCR was used. All of the lanes worked well and were bright and clear.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The flea had two bright bands that matched up with my positive controls and all the of the protocols were followed exactly. I’m confident the flea is positive for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Siphoneptera was found to be postive for Wolbachia.
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