|Captive / Cultivated?||Wild-caught|
|Group||Acton-Boxborough Regional High School|
The fungus gnat was collected/found in a patch of grass in between mowed and unmowed lawn, so there were some well-maintained lawn and some dryer, yellow grass with weeds. There were also very few leaves in the area, and it was fairly open. There was also mulch a few feet away from the area. It was around 10:00 AM when the gnat was collected. It was about 65º F and cloudy.
|Putative identification||Hexapoda Insecta Diptera Mycetophilidae|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Abdomen|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniOne|
|Explanation of confidence level||
The controls worked and were CO1 positive, which validates the quality of the wolbachia negative.
|Wolbachia 16S sequence||
|Arthropod COI sequence|| Download AB1
|Summary||The Mycetophilidae was found to be negative for Wolbachia.|