Fungus Gnat 3-3 Remote

November 10, 2020

Sample information
Picture
Photos by: Kabir M.
Location
Collection date 10/09/2020
Captive / Cultivated? Wild-caught
Group Acton-Boxborough Regional High School
Observations

The fungus gnat was collected/found in a patch of grass in between mowed and unmowed lawn, so there were some well-maintained lawn and some dryer, yellow grass with weeds. There were also very few leaves in the area, and it was fairly open. There was also mulch a few feet away from the area. It was around 10:00 AM when the gnat was collected. It was about 65ยบ F and cloudy.
Putative identification Arthropoda Hexapoda Insecta Diptera Mycetophilidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked and were CO1 positive, which validates the quality of the wolbachia negative.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Mycetophilidae was found to be negative for Wolbachia.
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