American Dog Ticks

November 18, 2020

Sample information
Picture
Photos by: Elijah D.
Location
Collection date 07/29/2020
Captive / Cultivated? Wild-caught
Group St. John High School USD 350
Observations

The ticks were collected off of my dog during the late summer. They had not yet burrowed into the skin.
Putative identification Arthropoda Chelicerata Arachnida Ixodida

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The ticks were stored in 90% ethanol for about three months prior to extraction.

DNA Extraction: The DNA was extracted from the abdomen.The abdomen was sliced open due to the tough exoskeleton. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.

PCR: Mini One Taq polymerase was used.

Gel Electrophoresis:  Mini PCR was used. All of the lanes worked well and were bright and clear.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of the controls worked and the bands were bright and clear. All of the protocols were followed exactly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Ixodida was found to be negative for Wolbachia.
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