Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 07/29/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | St. John High School USD 350 |
| Observations | The ticks were collected off of my dog during the late summer. They had not yet burrowed into the skin. |
| Putative identification | Arthropoda Chelicerata Arachnida Ixodida |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The ticks were stored in 90% ethanol for about three months prior to extraction. DNA Extraction: The DNA was extracted from the abdomen.The abdomen was sliced open due to the tough exoskeleton. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete. PCR: Mini One Taq polymerase was used. Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All of the controls worked and the bands were bright and clear. All of the protocols were followed exactly. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Ixodida was found to be negative for Wolbachia. |
Water Boatman