DKH 7

November 21, 2024

Sample information
Picture
Photos by: Dylan H.
Location
Collection date 08/29/2024
Captive / Cultivated? Wild-caught
Group Auburn University at Montgomery
Observations

A black carpenter ant found roaming in leaf litter.
Putative identification Arthropoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Wolbachia was positively identified by both electrophoresis and sequencing.
Wolbachia 16S sequence Download FASTA    Download AB1
GGTGTTGnnTGGCTGTCGTCnGCTCGTGTCGTGAGnnGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTT ACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCAT GGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCT TAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACG CCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TTGnNnnTTnnnCGGGnnnAATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACnATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGAACTATCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATnnTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCnnnTATTTCCCTAGATAAAATTCCCTTATTAGTATGATCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAnGnGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTnnTTnnTnnnnCnCTCnGGnnGnnGnnnnnnnAnTTnnTnCnnAnnTTTnnTTnnnnTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Formicidae was found to be postive for Wolbachia.
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