Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Auburn University at Montgomery |
| Observations | Ostracod with round body, yellow coloration with some darker brown areas. |
| Putative identification | Arthropoda Crustacea Ostracoda Podocopida |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | Ethidium Bromide |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | No Wolbachia DNA was detected during the gel, though sequencing quality was low which may indicate an error during extraction or PCR. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TnnNNNnnnnnnnnnnnnnnnTnnnnnnnnnnnnnnnnnnnnnnnTCnAnnnnAnTTnnnnnnnnnnnnnnnnnnnnnnn nNnnnGATCnnnnTnnnnnnnnnnnnnnnnnCnnnnnnTGCTTTTATTATAATTTTTTTTATAGTTATnCnnnnnATAAT TnnnnGATTTnnnnnnnnnnnnnTTCnnnnnnnnnnnnnAnnnnCTGATATAnCnnnnnnnnnnnnnAATAAnATAnnnn TnnnnnnnnnnCnnCCnnnnnnnnnnnTAnTnnnnnnnnnnnnnnnnnnnnnnnnnnGnnnnnGAACnnnnnnAAnnnnn nnnCCCCnnnnnnnnTCnAAnnnnnnnnnnnnnGGnnnnnnnnnnnnnnnnnnnnnnnnTnnnnnnnnnnnnnnnnGnnn nnnnnnnnTTnnnnnAnCnnnnnnnTTnnnnnnnnCnnTnATnAATATnnnnnnnnnNNNnnnnnnnnTnnAnnnnnnnn nnnnnnnnnnnnnAnnnnnnnnnnnTACAnnnnnnnnnNnnnnnnnnnnnnnnCnnGnnnnnnCnnGNnCTATTACnnTn nTnnnAACnnnnnnAAnTnTnnnnnnnnnnnTnnnnnnnnnnnnnnnnnnnnnAnnnnCnnnnnnnnnnnnnnnnTnnnT TnnnnTTTT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Podocopida was found to be negative for Wolbachia. |
Water Boatman