DKH8

November 21, 2024

Sample information
Picture
Photos by: Dylan H.
Location
Collection date
Captive / Cultivated? Wild-caught
Group Auburn University at Montgomery
Observations

Ostracod with round body, yellow coloration with some darker brown areas.
Putative identification Arthropoda Crustacea Ostracoda Podocopida

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

No Wolbachia DNA was detected during the gel, though sequencing quality was low which may indicate an error during extraction or PCR.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TnnNNNnnnnnnnnnnnnnnnTnnnnnnnnnnnnnnnnnnnnnnnTCnAnnnnAnTTnnnnnnnnnnnnnnnnnnnnnnn nNnnnGATCnnnnTnnnnnnnnnnnnnnnnnCnnnnnnTGCTTTTATTATAATTTTTTTTATAGTTATnCnnnnnATAAT TnnnnGATTTnnnnnnnnnnnnnTTCnnnnnnnnnnnnnAnnnnCTGATATAnCnnnnnnnnnnnnnAATAAnATAnnnn TnnnnnnnnnnCnnCCnnnnnnnnnnnTAnTnnnnnnnnnnnnnnnnnnnnnnnnnnGnnnnnGAACnnnnnnAAnnnnn nnnCCCCnnnnnnnnTCnAAnnnnnnnnnnnnnGGnnnnnnnnnnnnnnnnnnnnnnnnTnnnnnnnnnnnnnnnnGnnn nnnnnnnnTTnnnnnAnCnnnnnnnTTnnnnnnnnCnnTnATnAATATnnnnnnnnnNNNnnnnnnnnTnnAnnnnnnnn nnnnnnnnnnnnnAnnnnnnnnnnnTACAnnnnnnnnnNnnnnnnnnnnnnnnCnnGnnnnnnCnnGNnCTATTACnnTn nTnnnAACnnnnnnAAnTnTnnnnnnnnnnnTnnnnnnnnnnnnnnnnnnnnnAnnnnCnnnnnnnnnnnnnnnnTnnnT TnnnnTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Podocopida was found to be negative for Wolbachia.
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