Myrmica scabrinodis AB1

September 11, 2024

Sample information
Picture
Photos by: Laurin E.
Location
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

earthy forest path
Putative identification Arthropoda Insecta Hymenoptera Formicidae Myrmica Myrmica scabrinodis

Methods
Extraction kit Instagene Matrix
DNA extraction location
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Very confident, we always followed the instruction step-by-step.
Wolbachia 16S sequence Download FASTA    Download AB1
ACGAGCGCAACCCTCATCCTTAGWTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGYCGCGAGGCTAAGCTAATCCCTTAAAGGCCATCTCAGYTCGGATTGTACTCTGCWACTCGAGTGCATGAAGKTGGAATCGCTAGTMATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGYTTCACYTCKWWGCTATSYKTRGCGARSTRKGTATRRGTGCTATTAATTTTATCTCAACRATTA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CTTCAATAAGAATAATTATTCGTTTAGAATTAGGATCTTGTAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGGCTTTGGTAATTTTTTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Myrmica scabrinodis was found to be postive for Wolbachia.
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