Fly

September 20, 2024

Sample information
Picture
Photos by: Berry Ng
Location
Collection date 09/03/2024
Captive / Cultivated? Captive / Cultivated
Group Galileo Academy of Science and Technology
Observations

Black, 6 legs, 2 wings, 2 eyes, 2 antennaes
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit BABEC
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Taq polymerase

Annealing temperatures for each PCR reaction.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

the band was very clear.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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