False Widow Spider

September 20, 2024

Sample information
Picture
Location
Collection date 09/03/2024
Captive / Cultivated? Captive / Cultivated
Group Galileo Academy of Science and Technology
Observations
  • Beige colored
  • Black and white pattern on abdomen
  • 8 legs
  • 8 eyes
  • Hairy legs and abdomen
  • 2 mouth parts
Putative identification Arthropoda Chelicerata Arachnida

Methods
Extraction kit BABEC
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Taq polymerase was used.

Annealing temperature for each PCR reaction was 55 degrees celsius.

5th well starting from the left to right

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The insect DNA wasn’t very clear but the Wolbachia DNA was.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arachnida was found to be postive for Wolbachia.
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