Empoasca

October 22, 2024

Sample information
Picture
Photos by: Elin H.
Location
Collection date 08/20/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

I found it on a leaf of a hazelnut tree.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Cicadellidae Empoasca

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer
DNA stain Midori 1:20'000
Gel images
Protocol notes

This cicada didn‘t show any results on both PCRs. This might be due to an error in the DNA extraction or the PCR process. There is a possibility that some chemicals or bad conditions lead to the DNA degrading.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

There were no results in both PCRs. The second PCR was even done by the teacher.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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