philaenus spumarius

October 22, 2024

Sample information
Picture
Photos by: Elin H.
Location
Collection date 08/20/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

I found it on a leaf of a hazelnut tree.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Aphrophoridae Philaenus Philaenus spumarius

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer
DNA stain Midori 1:20'000
Gel images
Protocol notes

This sample showed the DNA in the final PCR. However, in our fist PCR no results showed. The DNA was still on top and didn‘t come down, the marker worked though. This might be due to an error in the process. Since it worked the second time, there has to be an error in the PCR process and not in the DNA extraction. That might be a wrong amount of a solution or the wrong order of steps.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The line in the PCR wasn‘t very strong and rather faint. Also, in our first PCR no line showed.

However, when we got back our DNA data and turned it into fasta files to find out the sequence, this organism had perfect quality of data.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Philaenus spumarius was found to be postive for Wolbachia.
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