Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/31/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | John Overton High School |
| Observations | Specimen was found under a flat rock with other crickets of the same species. |
| Putative identification | Arthropoda Insecta Orthoptera Gryllidae Gryllus Gryllus pennsylvanicus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | During PCR, a pipette tip was used to distribute PCR cocktail to multiple samples without being changed in between. This means that the samples may be cross-contaminated, but the controls show no evidence of this. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | The confidence level in this individual sample is low. While the DNA Ladder, (+) Arthropod control, and Water control appeared as expected, the (-) Arthropod control and (+) DNA control did not appear at all on the gel. Additionally, no distinct bands show up; only smudging. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid