Crane fly

January 4, 2021

Sample information
Picture
Photos by: Damian H
Location
Collection date 11/02/2020
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

Collected on misty morning from car door frame
Putative identification Arthropoda Insecta Diptera Tipulidae Tipula

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes
We first ran duplex PCR reaction (with both COI and Wolbachia primers) and then ran separate PCR reactions with just COI and just Wolbachia primers

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

High confidence level, the gel showed now Wolbachia band at the corresponding marker on the control lane
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
Summary The Tipula was found to be negative for Wolbachia.
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