Sample 1- Pavement Ant

December 20, 2020

Sample information
Picture
Photos by: E.C.
Location
Collection date 09/24/2020
Captive / Cultivated? Wild-caught
Group Hillsboro High School
Observations

The ant was easy to capture considering my hands are huge and it’s tiny. At first it tried to run away but I had sugar on my gloves and it seemed to be attracted to it. The ant was small, less then an inch small and had a thin black coat as the shell of the body.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system
Buffer Other
DNA stain
Gel images
Protocol notes

-Wolbachia positive

-Easy to see in gel image.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be postive for Wolbachia.
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