Bagworm Larvae

January 19, 2021

Sample information
Picture
Location
Collection date 08/13/2020
Captive / Cultivated? Wild-caught
Group St. John High School USD 350
Observations

The bagworms were found in their cases on cedar trees.
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The bagworms were removed from their cases and were stored in 90% ethanol for about five months prior to extraction.

DNA Extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.

PCR: Mini One Taq polymerase was used.

Gel Electrophoresis:  Mini PCR was used. All of the lanes worked well and were bright and clear.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The Wolbachia positive controls were two bright bands but the bagworms were single banded therefore I am confident that they are negative for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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