Team Matt Wolbachia project results

Sample information
Picture	Photos by: Matt H., Hunter S., Vasil B., Zyir V.
Location
Collection date	09/10/2024
Captive / Cultivated?	Wild-caught
Group	Owego Free Academy
Observations	Moths and grasshoppers alike are large arthropods with thick exoskeletons that should contain large amounts of DNA
Putative identification	Arthropoda
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes	In gel 2, lanes 4 and 5 had a small hole about half way down the gel, but this did not effect the results in any way
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	We are confident that we did the lab correctly because the control lines are all present. Also, whilst doing the procedures we were very careful and followed the steps very closely.
Wolbachia 16S sequence	detected in positive arthropod control and DNA controL BLAST at The Wolbachia Project BLAST at NCBI
Arthropod COI sequence	detected in all samples except water BLAST at The Wolbachia Project BLAST at NCBI
Summary	The Arthropoda was found to be negative for Wolbachia.

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The Wolbachia Project Database