MATAEF24-LY – Hempitera

November 5, 2024

Sample information
Picture
Photos by: Landon Y.
Location
Collection date 08/27/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

10:10 am, humid and sunny morning, caught in the high grass on the far east side of the student parking lot, caught by using the sweeping method.
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We are fairly certain that the insect we had caught, has zero traces of Wolbachia present within it. We know this because we performed a gel electrophoresis test and our results came back with only one band present in the insect gene, and nothing for the Wolbachia gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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