Wolf Spider – Wolbachia Project #2

November 14, 2024

Sample information
Picture
Photos by: Lilyana S.
Location
Collection date 11/01/2024
Captive / Cultivated? Wild-caught
Group Davies Career and Technical High School
Observations

Running fast trying to hide under leaves, near a fallen tree.
Putative identification Arthropoda Chelicerata Arachnida

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I’m confident because my Wolbachia lane for my Wolf Spider came out blank, but the fruit flies appeared, so the Taq Mater Mix worked my bug didn’t have any Wolbachia at all.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arachnida was found to be negative for Wolbachia.
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