Wolbachia Project #2 Ant

Sample information

Picture
Location
Collection date 11/01/2024
Captive / Cultivated? Wild-caught
Group Davies Career and Technical High School
Observations

The ant I collected was found in the woods in a decaying tree. It was similar to the Australian meat ant. I was unable to get a pictture of it.

Putative identification Arthropoda

Methods

Extraction kit DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level

These are my results and I got all of the bands that were supposed to appear. I am confident that the ant I did has Wolbachia

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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