Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | Segmented grey/brownish body Orange separating each segment Orange spots on each side and middle of each segment Cream colored legs |
| Putative identification | Arthropoda Diplopoda Polydesmida Euryuridae Euryurus Euryurus leachii |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Gonads dissected |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Lane 3 shows the positive results for the Wolbachia Gel Electrophoresis. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | In Lane 3 of the mini Gel, there was a band that is likely to be Wolbachia. The negative controls also showed positive for Wolbachia, so confidence is not that high. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Euryurus leachii was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid