Black Carpenter Ant

November 18, 2024

Sample information
Picture
Photos by: Soshi Kusanagi
Location
Collection date 11/14/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

It is black all-round the body with large mandibles.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Lane 12

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The confidence is not very high, due to the positive and negative controls not being accurate.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Camponotus pennsylvanicus was found to be negative for Wolbachia.
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