Cricket

March 2, 2021

Sample information
Picture
Location
Collection date 10/17/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Visible pellet
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Arthropod gel – controls didn’t work

Wolbachia gel- Controls and Wolbachia DNA didn’t work

 

 

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Issues with controls
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be postive for Wolbachia.
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