Portland State University – Lycosidae

November 25, 2024

Sample information
Picture
Location
Collection date 10/15/2024
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Chelicerata Arachnida Araneae Lycosidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Gel B, wells 8-10. Well 8 is positive for arthropod DNA, well 9 is positive for the Wolbachia positive control, well 10 is negative for Wolbachia in the spider.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lycosidae was found to be negative for Wolbachia.
Report Inappropriate Post