Portland State University – Musca domestica

Sample information

Picture
Photos by: Tiffany LaFountain
Location
Collection date 10/15/2024
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Hexapoda Insecta Diptera Muscidae Musca Musca domestica

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

Gel A, wells 11-13. Well 11 is positive for insect DNA, well 12 is positive for Wolbachia positive control, and well 13 is negative for Wolbachia in the fly.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Musca domestica was found to be negative for Wolbachia.
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