Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/31/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | North Penn-Mansfield JSHS |
| Observations | Location – Top of stream side, dirt new from flooding Notes – Many of same species around |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Nylanderia Nylanderia vividula |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | All controls returned as expected, test lane returned two very defined bands, but the farmost band from the loading well was not quite in line with the positive DNA control or positive arthropod control. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Nylanderia vividula was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
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Wolbachia data
Meadow Katydid