Roly Poly Wolbachia in Benu

December 4, 2024

Sample information
Picture
Photos by: Reem N
Location
Collection date 09/04/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2024 students by aspirator in grassy land by a pond
Putative identification Arthropoda Malacostraca Isopoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I had a weak Wolbachia band in my gel plate however I had a 100% BLAST match
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence Download FASTA    Download AB1
Summary The Isopoda was found to be postive for Wolbachia.
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