Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/04/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | The insect was caught near Lake Saint Benedict on a bush using a plastic jar. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | We cannot confirm the results due to DNA failure in PCR. We didn’t find DNA or Wolbachia in the gel electrophoresis run. We suspect that the pH levels could be influencing the results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
(no title)
Big Black Beetle
Fly
Roly Poly Wolbachia in Benu
Citrine Forktail (Ischnura hastata)