Sample information
Picture	Photos by: Aloha Escalante
Location
Collection date	11/12/2024
Captive / Cultivated?	Wild-caught
Group	Hawaii Community College
Observations	moved slowly when caught, couldn’t fly properly, red eyes
Putative identification	Arthropoda Hexapoda Insecta Diptera
Methods
Extraction kit	pcr
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TBE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Unknown
Confidence level	High
Explanation of confidence level	The DNA extraction was unsuccessful, only the DNA ladder is visible on the gel in lane 5
Wolbachia 16S sequence
Arthropod COI sequence
Summary