Ant

March 10, 2025

Sample information
Picture
Photos by: Langston T
Location
Collection date 09/19/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Black ant, with some lines on the third body section.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We had 2 controls that both worked, which means that our experiment worked; my lane of gel electrophoresis only had one line, which means there was no Wolbachia present.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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