Sample information
Picture	Entry by: Henrik B
Location	Approximate location: 44.93, -93.16
Collection date	09/19/2024
Captive / Cultivated?	Wild-caught
Group	St. Paul Academy and Summit School
Observations	Brown body, light brown legs
Putative identification	Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius neoniger
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Lasius neoniger was found to be negative for Wolbachia.