Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 09/16/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | Arthropod has three body segments (head, thorax, and abdomen) with six legs, 2 antennae, and a proboscis. It has a striped abdomen with its limbs having joints, two eyes with a slightly furry thorax, and legs. It was collected in the Fall in a house where the temperature was room temperature but it came from outside which is its normal physical habitat |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | First Gel Electrophoresis didn’t show results (My Arthropod DNA in Lane 6). The gel may have been punctured in the second trial in Lane 4 (My Arthropod DNA in Lane 8). |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Arthropod DNA is present, but no Wolbachia DNA is present. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Culicidae was found to be negative for Wolbachia. |
Flying Carpenter Ant
Mr Spider
Roly Poly
Ant
ANT