Citrine Forktail (Ischnura hastata)

December 4, 2024

Sample information
Picture
Entry by: Matthew Z.
Location
Collection date 09/04/2024
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

The insect was caught near Lake Saint Benedict on a bush using a plastic jar.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

We cannot confirm the results due to DNA failure in PCR. We didn’t find DNA or Wolbachia in the gel electrophoresis run. We suspect that the pH levels could be influencing the results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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