Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/31/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | North Penn-Mansfield JSHS |
| Observations | Location – Moist dirt in thick brush, on small stick Notes – Dangling from silk |
| Putative identification | Arthropoda Arachnida Araneae Salticidae Naphrys Naphrys pulex |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Negative arthropod control returned two lines, and the Wolbachia DNA retuned just one. While the test lane shows a very clear single line, the unexpected control results mean the test lane is untrustworthy. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Naphrys pulex was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid