Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Hawaii Community College |
| Observations | moved slowly when caught, couldn’t fly properly, red eyes |
| Putative identification | Arthropoda Insecta Diptera |
Methods |
|
| Extraction kit | pcr |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Lane 1 Arthropod PCR of Insect 1 Lane 2 Arthropod PCR of Insect 2 Lane 3 Wolbachia PCR of Insect 1 Lane 4 Wolbachia PCR of Insect 2 Lane 5 DNA ladder
|
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | The DNA extraction was unsuccessful, only the DNA ladder is visible on the gel in lane 5 |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid