Big Black Beetle

December 4, 2024

Sample information
Picture
Photos by: Lori H.
Location
Collection date 11/11/2024
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations

– The beetle was quite large and robust
– It had a dark outer hard shell with white speckles
– It had wings and was able to fly, but very slowly
– I found it eating my compost
Putative identification Arthropoda Hexapoda Insecta Coleoptera Scarabaeidae Amithao Amithao decemguttatus

Methods
Extraction kit pcr
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There was only a result in the Arthropod CO1 DNA, no Wolbachia showed up on the gel results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Amithao decemguttatus was found to be negative for Wolbachia.
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