Ant

December 5, 2024

Sample information
Picture
Photos by: Andre E.
Location
Collection date 11/14/2024
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations
Putative identification Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius niger

Methods
Extraction kit pcr
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Gel Lanes (Top gel)

  1. DNA ladder
  2. Sample 1 CO1
  3. Sample 2 CO1
  4. Sample 1 16S
  5. Sample 2 16S

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I believe for one of the samples were ruined, hence why the first DNA sample didn’t appear. But I still believe that it is enough information to show that these specific garden ants don’t have wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lasius niger was found to be negative for Wolbachia.
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