Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/07/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | John Bapst Memorial High School |
| Observations | Black Fly |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | The results for the Black Fly are in the second set of wells titled 1-6. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Since the DNA was not correctly extracted and the lines in the Wolbachia wells were higher than they should have been, levels of confidence for this procedure are low. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Bombus impatients (Eastern Bumblebee)
Agelenopis
My Data
Common Eastern Bumble Bee (Bombus impatiens)
American Bird