spider Aviv Goldstein and Odel Hillel

February 4, 2025

Sample information
Picture
Photos by: Odel Hillel
Location
Collection date 12/25/2024
Captive / Cultivated? Wild-caught
Group Katzir High School
Observations

It was alive found on a wall
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Caught the spider with our hands
Place it in a Ethanol-filled test tube
DNA extraction process
DNA replication using PCR machine
electrophoresis

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

there was no band for the Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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